In general spots can be expected to be unreliable if they are very small, or
very large relative to the other spots in the array. This could be due
to problems in the washing process of the array, or bleeding at a
spot. The shape of the
spot is also a measure, and if they are markedly non-circular, you can
draw the same conclusion as for the size. A measurement concerning the
size is given by
where
is the number of pixels per spot, and
is the average
number of pixels per spot.
Bjørn Kåre Alsberg
2006-04-06