Sometimes the intensity of a pixel exceeds the detection
range of the scanner, i.e
, this effect is called saturation.
It i common to reject all spots
that have more than
of its pixels saturated if mean intensity
values are used, while you only reject spots with more than
saturated pixels if the median is used, since it is less sensitive to
variation [5].
Bjørn Kåre Alsberg
2006-04-06