loess

Intensity-dependent normalisation, or global loess normalisation, does a loess fit based on all the values in the MA-plot

$\displaystyle M=M-$loess$\displaystyle (A)$    

This results in a smoothening of the image, and removes unwanted features concerning the different characteristics in the red and green dye.

Bjørn Kåre Alsberg 2006-04-06