The RMA normalisation routine performs a quantile normalisation,
[1]. This is a non-parametric procedure that works as
follows:
- Compute the quantile of each value in the distribution of probe
intensities.
- Transform the original value to that quantiles value on the
reference chip:
where
is the distribution function of chip
, and
is the distribution function of the reference chip.
One strong assumption made in this procedure is that the distribution
of probe intensities are identical.
Bjørn Kåre Alsberg
2006-04-06